Identification of Naegleria fowleri proteins linked to primary amoebic meningoencephalitis

Naegleria fowleri (N. fowleri) causes primary amoebic meningoencephalitis, a rapidly fatal disease of the central nervous system. N. fowleri can exist in cyst, flagellate or amoebic forms, depending on environmental conditions. The amoebic form can invade the brain following introduction into the nasal passages. When applied intranasally to a mouse model, cultured N. fowleri amoebae exhibit low virulence. However, upon serial passage in mouse brain, the amoebae acquire a highly virulent state. In the present study, a proteomics approach was applied to the identification of N. fowleri amoeba proteins whose expression was associated with the highly virulent state in mice. Mice were inoculated intranasally with axenically cultured amoebae or with mouse-passaged amoebae. Examination by light and electron microscopy revealed no morphological differences. However, mouse-passaged amoebae were more virulent in mice as indicated by exhibiting a two log10 titre decrease in median infective dose 50 (ID50). Scatter plot analysis of amoebic lysates revealed a subset of proteins, the expression of which was associated with highly virulent amoebae. MS-MS indicated that this subset contained proteins that shared homology with those linked to cytoskeletal rearrangement and the invasion process. Invasion assays were performed in the presence of a select inhibitor to expand on the findings. The collective results suggest that N. fowleri gene products linked to cytoskeletal rearrangement and invasion may be candidate targets in the management of primary amoebic meningoencephalitis

The evolution of Giant Molecular Filaments

In recent years there has been a growing interest in studying giant molecular filaments (GMFs), which are extremely elongated (> 100pc in length) giant molecular clouds (GMCs). They are often seen as inter-arm features in external spiral galaxies, but have been tentatively associated with spiral arms when viewed in the Milky Way. In this paper, we study the time evolution of GMFs in a high-resolution section of a spiral galaxy simulation, and their link with spiral arm GMCs and star formation, over a period of 11Myrs. The GMFs generally survive the inter-arm passage, although they are subject to a number of processes (e.g. star formation, stellar feedback and differential rotation) which can break the giant filamentary structure into smaller sections. The GMFs are not gravitationally bound clouds as a whole, but are, to some extent, confined by external pressure. Once they reach the spiral arms, the GMFs tend to evolve into more substructured spiral arm GMCs, suggesting that GMFs may be precursors to arm GMCs. Here, they become incorporated into the more complex and almost continuum molecular medium that makes up the gaseous spiral arm. Instead of retaining a clear filamentary shape, their shapes are distorted both by their climb up the spiral potential and their interaction with the gas within the spiral arm. The GMFs do tend to become aligned with the spiral arms just before they enter them (when they reach the minimum of the spiral potential), which could account for the observations of GMFs in the Milky Way.Comment: 15 pages, 11 figures, MNRAS accepte

Extrapolation for classes of weights related to a family of operators and applications

In this work we give extrapolation results on weighted Lebesgue spaces for weights associated to a family of operators. The starting point for the extrapolation can be the knowledge of boundedness on a particular Lebesgue space as well as the boundedness on the extremal case L∞. This analysis can be applied to a variety of operators appearing in the context of a Schrödinger operator ( −Δ + V) where V satisfies a reverse Hölder inequality. In that case the weights involved are a localized version of Muckenhoupt weights.Fil: Bongioanni, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Matemática Aplicada del Litoral. Universidad Nacional del Litoral. Instituto de Matemática Aplicada del Litoral; ArgentinaFil: Cabral, A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Matemática Aplicada del Litoral. Universidad Nacional del Litoral. Instituto de Matemática Aplicada del Litoral; ArgentinaFil: Harboure, Eleonor Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Matemática Aplicada del Litoral. Universidad Nacional del Litoral. Instituto de Matemática Aplicada del Litoral; Argentin

Some Problems on the Classical N-Body Problem

Our idea is to imitate Smale's list of problems, in a restricted domain of mathematical aspects of Celestial Mechanics. All the problems are on the n-body problem, some with different homogeneity of the potential, addressing many aspects such as central configurations, stability of relative equilibrium, singularities, integral manifolds, etc. Following Steve Smale in his list, the criteria for our selection are: (1) Simple statement. Also preferably mathematically precise, and best even with a yes or no answer. (2) Personal acquaintance with the problem, having found it not easy. (3) A belief that the question, its solution, partial results or even attempts at its solution are likely to have great importance for the development of the mathematical aspects of Celestial Mechanics.Comment: 10 pages, list of mathematical problem

Role of heat generation and thermal diffusion during frontal photopolymerization

Frontal photopolymerization (FPP) is a rapid and versatile solidification process that can be used to fabricate complex three-dimensional structures by selectively exposing a photosensitive monomer-rich bath to light. A characteristic feature of FPP is the appearance of a sharp polymerization front that propagates into the bath as a planar traveling wave. In this paper, we introduce a theoretical model to determine how heat generation during photopolymerization influences the kinetics of wave propagation as well as the monomer-to-polymer conversion profile, both of which are relevant for FPP applications and experimentally measurable. When thermal diffusion is sufficiently fast relative to the rate of polymerization, the system evolves as if it were isothermal. However, when thermal diffusion is slow, a thermal wavefront develops and propagates at the same rate as the polymerization front. This leads to an accumulation of heat behind the polymerization front which can result in a significant sharpening of the conversion profile and acceleration of the growth of the solid. Our results also suggest that a novel way to tailor the dynamics of FPP is by imposing a temperature gradient along the growth directio

Filamentary fragmentation in a turbulent medium

We present the results of smoothed particle hydrodynamic simulations investigating the evolution and fragmentation of filaments that are accreting from a turbulent medium. We show that the presence of turbulence, and the resulting inhomogeneities in the accretion flow, play a significant role in the fragmentation process. Filaments which experience a weakly turbulent accretion flow fragment in a two-tier hierarchical fashion, similar to the fragmentation pattern seen in the Orion Integral Shaped Filament. Increasing the energy in the turbulent velocity field results in more sub-structure within the filaments, and one sees a shift from gravity-dominated fragmentation to turbulence-dominated fragmentation. The sub-structure formed in the filaments is elongated and roughly parallel to the longitudinal axis of the filament, similar to the fibres seen in observations of Taurus, and suggests that the fray and fragment scenario is a possible mechanism for the production of fibres. We show that the formation of these fibre-like structures is linked to the vorticity of the velocity field inside the filament and the filament's accretion from an inhomogeneous medium. Moreover, we find that accretion is able to drive and sustain roughly sonic levels of turbulence inside the filaments, but is not able to prevent radial collapse once the filaments become supercritical. However, the supercritical filaments which contain fibre-like structures do not collapse radially, suggesting that fibrous filaments may not necessarily become radially unstable once they reach the critical line-density.Comment: (Accepted for publication in MNRAS